Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow...

Effects of various cryoprotectants on the survival of mouse embryos cryopreserved by the quick freezing method.

The effects of concentrations of glycerol, ethylene glycol or dimethylsulphoxide (DMSO) in the presence of either 0.25 M lactose or sucrose on the post-thaw survival of mouse quickly-frozen compacted morulae were studied. In this method, the embryos were directly frozen in liquid nitrogen (LN2) vapor at approximately -170 degrees C for 2 min before being plunged into LN2. High survival rates of...

In vitro survival of in vitro-produced bovine embryos cryopreserved by slow freezing, fast freezing and vitrification

In order to compare the survival rate of bovine embryos cryopreserved with different protocols, oocytes obtained from abattoir-derived ovaries were used for in vitro maturation (IVM), fertilization (IVF) and co-culture. Expanded blastocysts were cryopreserved by two slowfreezing [(i) 0.5 and (ii) 1.2°C/min], (iii) one quickfreezing and two vitrification [(iv) ethylene glycol + ficoll + sucrose ...

I-11: Cryopreservation of Canine Embryos

Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...

Embryo donation: unresolved legal issues in the transfer of surplus cryopreserved embryos.